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fixable viability dye efluor 450 thermo fisher scientific  (Thermo Fisher)


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    Thermo Fisher fixable viability dye efluor 450 thermo fisher scientific
    Fixable Viability Dye Efluor 450 Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixable+viability+dye+efluor+450+thermo+fisher+scientific/pm34767747-292-34-39
    Average 86 stars, based on 1 article reviews
    fixable viability dye efluor 450 thermo fisher scientific - by Bioz Stars, 2026-09
    86/100 stars

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    Staining:

    Article Title: MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 ..

    Methylation:

    Article Title: MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 ..

    Virus:

    Article Title: MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 ..

    Modification:

    Article Title: MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 ..

    Sequencing:

    Article Title: MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 ..

    Real-time Polymerase Chain Reaction:

    Article Title: MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 ..

    Recombinant:

    Article Title: MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 ..

    Plasmid Preparation:

    Article Title: MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 ..

    Nuclear Magnetic Resonance:

    Article Title: MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beads Beckman Coulter Cat# #A63881 Epredia Richard-Allan Scientific Three-Step Stain Kit Thermo Fisher Scientific Cat# 22-050-272 Ghost Dye Red 780 Viability Dye Cell Signaling Technology Cat# 18452 Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific Cat# L34957 M-Digestion Buffer (2X) Zymo Research Cat# D5020-9 EZ-96 DNA Methylation-Direct Kit Zymo Research Cat# D5022 HotStarTaq Master Mix Kit QIAGEN Cat# 203443 Ion 520 & Ion 530 ExT Kit-Chef Thermo Fisher Scientific Cat# A30670 Bacterial and virus strains ElectroMAX DH10B Cells Thermo Fisher Scientific Cat#: 18290015 Oligonucleotides Array primers: Forward – TAACTTGAAAGTATTTCGAT TTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC ACCGReverse - ACTTTTTCAAGTTGATAACGGACTA GCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC Shalem et al., 2014 N/A Adaptor primers: Forward – AATGGACTATCATATG CTTACCGTAACTTGAAAGTATTTCGReverse - ACGG CATCGCAGCTTGGATACA Modified from Shalem et al. (2014) N/A Sequencing primers: Forward – AATGATACGGCG ACCACCGAGATCTACACTCTTTCCCTACACGACGC TCTTCCGATCTNNNNNNNNNTCTTGTGGAAAGGAC GAAACACCGReverse - CAAGCAGAAGACGGCA TACGAGATXXXXXXXXGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTACGGCATCGCAGCTTG GATACA Modified from Shalem et al. (2014) N/A 1C metabolism gRNA library This paper N/A TruSeq DNA UD Indexes IDT Cat# 20020590 MTHFD2 (human) qPCR primers:Forward - GGGAAGAATGTGGTTGTGGCReverse - ATGACTGCTGCTCCTTCCTT This paper N/A GAPDH (human) qPCR primers:Forward – TGATGACATCAAGAAGGTGGTGAAGReverse - TCCTTGGAGGCCATGTGGGCCAT N/A Recombinant DNA pMx-U6-empty-GFP plasmid (Toffalini et al., 2009) N/A MTHFD2-set siRNA/shRNA/RNAi Lentivector (Human) Applied Biological Materials Cat# 308880910296 Scrambled siRNA GFP LentivectorTarget Sequence - GGGTGAACTCACGT CAGAA Applied Biological Materials Cat# LV015-G pCMV-VSV-G Addgene Cat# 8454; RRID:Addgene_8454 psPAX2 Addgene Cat# 12260; RRID:Addgene_12260 Software and algorithms Prism v9 GraphPad Software https://www.graphpad.com; RRID: SCR_002798 FlowJo v10 FlowJo https://www.flowjo.com; RRID:SCRJD08520 El-MAVEN v0.8.0 Elucidata https://resources.elucidata.io/ elmaven#resources Chenomx NMR Suite v8.4 Chenomx https://www.chenomx.com MAGeCK v0.5.0.3 Li et al., 2014 N/A Trimmomatic v0.39 N/A (Continued on next page) Immunity 55, 65–81.e1–e9, January 11, 2022 e4 ..



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